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KMID : 0861020110260040149
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.149 ~ p.154
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Protective Effect of Cortex Fraxini on Heart Injury in a Rat Model of Myocardial Infarction
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Lim Sun-Ha
Lee Jong-Won
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Abstract
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Objectives: Myocardial infarction is caused by heart cell death in a region where coronary arteries supplying
blood to the region are occluded. In the present study, we determined whether ethanol extract of Cortex fraxini
(HY5053) could attenuate heart injury by inhibiting apoptosis.
Methods: Improvement of survival of HepG2 cells, a human hepatocellular carcinoma cell line, and reduction of
apoptosis under hypoxic conditions (3% O2) were assessed by trypan blue staining and DNA fragmentation
assay, respectively. To assess the impact of HY5053 on the heart injury, Sprague-Dawley rats underwent 1 day
of the left anterior descending coronary artery occlusion. HY5053 was given by intraperitoneal injection (200
mg/kg) 1 hr prior to the occlusion. Subsequently, the heart were harvested, excised into 4 slices, and the slices
were stained with 2,3,5-triphenyl tetrazolium chloride. Finally, the extent of heart injury represented as
ischemic index (%) was assessed.
Results: Addition of HY5053 (400 ㎍/mL) into the culture medium for 1 day under ischemic conditions improved
the cell survival by 50%, compared with control (0 ㎍/mL), consequently delayed apoptosis in 6 hr difference.
Also, HY5053 (200 mg/kg) reduced the ischemic index by 44%, compared with control (0 mg/kg).
Conclusions: These findings suggested that HY5053 attenuated myocardial infarction by inhibiting apoptosis.
Thus, Cortex fraxini could be developed as a novel cardioprotectant to complement a currently available
treatment, coronary angioplasty.
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KEYWORD
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Cortex fraxini, myocardial infarction, apoptosis, cardioprotectant
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